Heba Fawzy; Doaa M. Hendawy; Mohammed S. Ghareeb; Mahmoud Amer; Hend Sameh; Hala Mosaad
Abstract
Coronary artery disease (CAD) refers to the progressive development of atherosclerosis, which is a global significant mortality cause, initial diagnosis of CAD is a challenge. Consequently, ...
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Coronary artery disease (CAD) refers to the progressive development of atherosclerosis, which is a global significant mortality cause, initial diagnosis of CAD is a challenge. Consequently, biomarkers are required for prediction of subclinical atherosclerosis and early treatment. Previously, miR-126 and miR-423-3p were approved to be participants in (CAD) development. Yet, their association with subclinical atherosclerosis is unidentified. The aim of our study is to investigate the role of plasma miRNA 126 and miRNA 423-3p levels to predict subclinical atherosclerosis and correlate it with exercise ECG test. The levels of miR-126 and 423-3p were assessed in plasma from 50 healthy control (group I) and 50 subclinical atherosclerotic CAD cases (group II) by quantitative real time –PCR (qRT-PCR). Exercise ECG was done to detect ischemic changes in both groups. There was a significant decrease of miRNA 126 and miR 423-3p levels in group II compared to group I. Likewise, a positive correlation was detected between both miR 126 and miR 423-3p with positive exercise ECG test. ROC curves of both miR 126 and miR-423-3p to detect positive exercise ECG test showed sensitivity and specificity of 92.2% and 93.9%, correspondingly. The combination of both miR-126 and miR-423-3p is considered as hopeful indicators of subclinical atherosclerosis in risky individuals, thus intensifying the management of hazard factors might decrease atherosclerotic progression.
